Hi Guys
I testing distinct conversors from solexa fastq to standard sanger but have a couple of naive questions to do om which i am unclear
I think that as illumina 1.8+ encoding scheme encodes that Phred-score by adding 33 to the score there is no need of conversion as this is sanger too
Can anyone tell me if I am correct??
As for illumina 1.5+ and 1.8+
I donwloaded the fq_all2std script from maq and because in the source file the parameter in for loop aare -64 to 64 and the remaining parameters wherever for the conversion is again 64 i think this file is for illumina 1.3+
Am i right here??
Additonally if want to this script to convert from illumina 1.5+ to sanger is it enough to just change 64 wherever noted for 66?? Is that right? I mean is it just as simple as it seems or i am missing something in this last step?
thank you in advance
I testing distinct conversors from solexa fastq to standard sanger but have a couple of naive questions to do om which i am unclear
I think that as illumina 1.8+ encoding scheme encodes that Phred-score by adding 33 to the score there is no need of conversion as this is sanger too
Can anyone tell me if I am correct??
As for illumina 1.5+ and 1.8+
I donwloaded the fq_all2std script from maq and because in the source file the parameter in for loop aare -64 to 64 and the remaining parameters wherever for the conversion is again 64 i think this file is for illumina 1.3+
Am i right here??
Additonally if want to this script to convert from illumina 1.5+ to sanger is it enough to just change 64 wherever noted for 66?? Is that right? I mean is it just as simple as it seems or i am missing something in this last step?
thank you in advance
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