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Old 08-19-2015, 12:00 PM   #1
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Location: Sarajevo, Bosnia and Herzegovina

Join Date: Aug 2015
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Default Low final library

Hi everyone! I am brand new Ion Torrent user, and I tried to prepare a run for inherited disease panel. In the end, I got a very poor Final Library result,only 3% in which I see that most of the unusable reads were test fragments, 82%.Here is the screenshot.

Can You please tell me possible reasons for this?
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Old 09-10-2015, 10:07 PM   #2
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Location: Japan

Join Date: Jun 2015
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Could you please tell me your i)amount and size distribution of input DNA for library preparation, ii)final library images by bioanalyzer, and iii)library kit.
jon123 is offline   Reply With Quote

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