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Old 08-19-2015, 11:00 AM   #1
molpath
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Location: Sarajevo, Bosnia and Herzegovina

Join Date: Aug 2015
Posts: 1
Default Low final library

Hi everyone! I am brand new Ion Torrent user, and I tried to prepare a run for inherited disease panel. In the end, I got a very poor Final Library result,only 3% in which I see that most of the unusable reads were test fragments, 82%.Here is the screenshot.
[IMG]/Users/Riko/Desktop/5b37c3eba942a8df384012e4c200dd8d0b4a601f70d2e2296f707f1a11d8a234.0.jpg[/IMG]

Can You please tell me possible reasons for this?
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Old 09-10-2015, 09:07 PM   #2
jon123
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Location: Japan

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Could you please tell me your i)amount and size distribution of input DNA for library preparation, ii)final library images by bioanalyzer, and iii)library kit.
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