SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: RSEQtools: A modular framework to analyze RNA-Seq data using compact, anonym Newsbot! Literature Watch 1 02-27-2013 04:16 AM
RNA-Seq: Detection of splicing events and multiread locations from RNA-seq data based Newsbot! Literature Watch 0 10-26-2011 03:50 AM
RNA-Seq: Length Bias Correction for RNA-seq Data in Gene Set Analyses. Newsbot! Literature Watch 0 01-22-2011 03:02 AM
RNA-Seq: SAW: A Method to Identify Splicing Events from RNA-Seq Data Based on Splicin Newsbot! Literature Watch 0 08-14-2010 03:00 AM
RNA-Seq data set? simon8udke RNA Sequencing 1 04-15-2010 01:20 PM

Reply
 
Thread Tools
Old 04-18-2011, 04:38 PM   #1
Hunny
Member
 
Location: Beijing, China

Join Date: Apr 2011
Posts: 23
Default Where could I get a set of real RNA-Seq data

Hello guys!

I would like to know where I could get a set of real RNA-Seq data.

I've searched many papers about RNA-Seq, but I haven't found any one providing RNA-Seq data.

Thanks
Hunny is offline   Reply With Quote
Old 04-18-2011, 04:58 PM   #2
d17
Member
 
Location: United States

Join Date: Sep 2008
Posts: 27
Default

Try taking a look at this paper:
http://www.nature.com/nature/journal...ture08872.html

Their data is available on their website and it is pretty easy to access.
d17 is offline   Reply With Quote
Old 04-18-2011, 05:05 PM   #3
Hunny
Member
 
Location: Beijing, China

Join Date: Apr 2011
Posts: 23
Default

Quote:
Originally Posted by d17 View Post
Try taking a look at this paper:
http://www.nature.com/nature/journal...ture08872.html

Their data is available on their website and it is pretty easy to access.
Thank you so much. I will check it
Hunny is offline   Reply With Quote
Old 04-18-2011, 06:21 PM   #4
Hunny
Member
 
Location: Beijing, China

Join Date: Apr 2011
Posts: 23
Default

Quote:
Originally Posted by d17 View Post
Try taking a look at this paper:
http://www.nature.com/nature/journal...ture08872.html

Their data is available on their website and it is pretty easy to access.
Hi,

Thank you again! I have downloaded the data from their website successfully!

Since I want to test TopHat using a real dataset, I also need a reference genome for mapping the RNA-Seq data above.

So, How could I get a reference genome?

Thanks
Hunny is offline   Reply With Quote
Old 04-18-2011, 07:47 PM   #5
jstjohn
Member
 
Location: San Francisco, CA

Join Date: Jun 2010
Posts: 35
Default

http://www.ncbi.nlm.nih.gov/projects...an/index.shtml
jstjohn is offline   Reply With Quote
Old 04-19-2011, 12:57 PM   #6
wuj
Junior Member
 
Location: Long island

Join Date: Jul 2009
Posts: 8
Default

Quote:
Originally Posted by Hunny View Post
Hi,

Thank you again! I have downloaded the data from their website successfully!

Since I want to test TopHat using a real dataset, I also need a reference genome for mapping the RNA-Seq data above.

So, How could I get a reference genome?

Thanks
You need to get bowtie first, and....perhaps you need to read manuals of bowtie and tophat before you actually start....

FYI: reference genome sequences are available on UCSC genome browser -> download
wuj is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:53 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO