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Old 04-02-2014, 06:51 AM   #1
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Location: Germany

Join Date: Sep 2012
Posts: 8
Default Species specific primers

Hi All,

I need to design primer of one specific group of bacteria. For this i took all the sequences from that group and then did Multiple sequence alignment to find conserved region but these conserved region has Gaps. But still i pick chunk of sequences looked conserved and put it in probe check to see where its showing hits
Also I used these chunks(removed gaps) and put it in silva probe test to see the candidate primers for coverage.
I m not sure if i am doing it correctly and if so then how can I confirm my primers binding. Your inputs are valuable.

Thanks in advance

Last edited by naman; 04-02-2014 at 07:00 AM.
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