Originally posted by hmaa
View Post
- Generate the data base (consensus sequence) with Samtools tool
De novo in theory would work, but in practice, is a huge job for a mammalian transcriptome. And are you sure that your virus in integrated such that it makes a fusion transcript? Isn't it possible that it's just hanging out in intergenic space?
Your data seems to be telling you that you have no insertions of the kind you want. Is it really so very unlikely that that's not correct?
Comment