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Old 03-26-2014, 03:44 AM   #1
laurakrol
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Location: Utrecht

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Default Primer sequences emPCR and sequencing primer Ion Torrent

Hi,

Does anyone know the exact sequences of the primers that are used for the clonal amplification with the Ion PGM™ Template OT2 400 Kit? In addition I like to know what the sequence is of the sequencing primer used in the Ion PGM™ Sequencing 400 Kit?
I like to know exactly where the primers bind to the A and P1 adaptors.
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Old 04-25-2014, 05:08 AM   #2
Fernanda
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Laura, there's a good tech note about it (attached) and also this answer at life.com "The sequences can also be found on page 68 of the Ion Xpress™ Plus gDNA Fragment Library Preparation user guide. - See more at: http://ioncommunity.lifetechnologies.com/message/21338#21338"
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File Type: pdf ION_Amplicon sequencing.pdf (1.14 MB, 136 views)
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