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  • Gene sequence from transcript sequence

    I have variation information for SNPs and small indels together with the position of the variation relative to the transcript. I also have the transcript sequence because i have the NM_ID of the transcript. Two questions:
    1. Given the above, how do i obtain the position of the variation relative to the gene.
    2. How do i handle cases where the transcript sequence has been 'updated' by NCBI as compared to the transcript sequence data i have.(do an alignment of new transcript sequence data with earlier transcript sequence data?)

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