Hi All,
I need to design primer of one specific group of bacteria. For this i took all the sequences from that group and then did Multiple sequence alignment to find conserved region but these conserved region has Gaps. But still i pick chunk of sequences looked conserved and put it in probe check to see where its showing hits
Also I used these chunks(removed gaps) and put it in silva probe test to see the candidate primers for coverage.
I m not sure if i am doing it correctly and if so then how can I confirm my primers binding. Your inputs are valuable.
Thanks in advance
I need to design primer of one specific group of bacteria. For this i took all the sequences from that group and then did Multiple sequence alignment to find conserved region but these conserved region has Gaps. But still i pick chunk of sequences looked conserved and put it in probe check to see where its showing hits
Also I used these chunks(removed gaps) and put it in silva probe test to see the candidate primers for coverage.
I m not sure if i am doing it correctly and if so then how can I confirm my primers binding. Your inputs are valuable.
Thanks in advance