Hi,
recently several vendors are offering library prep kits which carry out end-repair and A-tailing without buffer change (simply using a two temperature incubation). The kits do not even require a buffer change/cleanup for the ligation. Please see the links below.
Would you have any idea which enzymes are used in these kits for A-tailing?
recently several vendors are offering library prep kits which carry out end-repair and A-tailing without buffer change (simply using a two temperature incubation). The kits do not even require a buffer change/cleanup for the ligation. Please see the links below.
Would you have any idea which enzymes are used in these kits for A-tailing?
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