Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Mitochondrial heteroplasmy level detection

    Hi, all

    We are currently study mitochondrial genomics, however, find it confusing when try to detect the heteroplasmy level. Can some explain how to detect the heteroplasmy level? Some one said, this level is equal to minor allele frequency, however if it is true, none of the heteroplasmy would be over 50%, which is certainly not true.

    Thanks in advance to whom answered my question.

  • #2
    Heteroplasmy can't exceed 50% unless the analysis is done at a haplotype level (i.e. considering multiple variants). For a single bimorphic variant, a heteroplasmy of greater than 50% would imply that the "other" variant was the dominant allele (and therefore heteroplasmy of less than 50%).

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    34 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 08:48 AM
    0 responses
    28 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-01-2024, 06:45 AM
    0 responses
    45 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-27-2024, 06:37 PM
    0 responses
    32 views
    0 likes
    Last Post seqadmin  
    Working...
    X