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Old 02-10-2017, 07:32 AM   #1
AlexClop
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Location: Catalonia

Join Date: Feb 2017
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Default Recommended pipeline for differential miRNA-seq

Dear all,

I was a SeqAnswers member few years ago while working on human genetics at Kings' College London. I just re-registered again, in my current job as animal geneticist at CRAG (Barcelona).

We want to carry a microRNA-seq experiment in an Illumina HiSeq2000 to carry a differential expression analysis from porcine sperm.

I would like to know which is the most recommendable bioinformatics pipeline nowadays for our miRNA-seq study.

Thanks for sharing your knowledge and experience!

Alex Clop
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Old 03-08-2017, 11:22 PM   #2
sbarberan
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I use CAP-miRSeq that includes differential expression analysis with edgeR

http://bioinformaticstools.mayo.edu/...ch/cap-mirseq/
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bioinformatic analysis, differential expression, mirna-seq, pipeline

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