Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • coverageBed -split giving incorrect output?

    In the command below, coverageBed seems to be ignoring the -split argument.

    $ coverageBed -split -a a2.bed -b b2.bed > out.txt

    (The inputs and the output for the command above are shown below in addition to being attached.)

    Please note that b2.bed defines two blocks, each of width 1960, and that a2.bed defines a single block of length 50 that is between those two blocks and does not intersect them. Yet, in out.txt the 13th field has a 1 in it, indicating that there was an overlap. Also, the 15th field in out.txt is the distance from the beginning of the first block of b2.bed to the end of the last block of b2.bed. I think it should be 1960+1960.

    What am I doing wrong? Any help appreciated.
    I'm running v2.12.0 of coverageBed.
    Thanks. -Ben

    $ cat a2.bed
    chrX 5561936 5561986 56_1638_1187_F3 50 - 5561936 5561986 255,0,01 50 0

    $ cat b2.bed
    chrX 4074963 30247441 eg:668963:chrX 0 + 4074963 4076923 0 2 1960,1960, 0,26170518,

    $ cat out.txt
    chrX 4074963 30247441 eg:668963:chrX 0 + 4074963 4076923 0 2 1960,1960, 0,26170518, 1 50 26172478 0.0000019
    Attached Files

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
39 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
41 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
35 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X