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  • gene ratios across RNASeq/amplicon datasets

    Say you have one set of data that is derived from isolating certain transcripts and amplifying them. And another set of data that consists of RNASeq of the entire transcriptome.

    Would you expect that the ratio between any two genes in a sample would be roughly similar across all samples from either set of samples?

    This is of course assuming that it's all from the same type of tissue, and that all the RNASeq data has been normalized. Basically, I'm trying to get my head around whether it is possible to compare amplicon data with data about the same genes extracted from RNASeq data.

    Cheers
    Ben.

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