Hi,
I have an illumina single read sequencing with read length 36.
By default, tophat cuts segments of 25 bases.
How does tophat cut reads of 36 bases in segments of 25 bases?
Do I need to change "--segment-length" option to 18?
Can you help me to find the best parameters for single-end reads of 36 bases?
Thanks,
Ickou
I have an illumina single read sequencing with read length 36.
By default, tophat cuts segments of 25 bases.
How does tophat cut reads of 36 bases in segments of 25 bases?
Do I need to change "--segment-length" option to 18?
Can you help me to find the best parameters for single-end reads of 36 bases?
Thanks,
Ickou
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