Hi,
I was wanting to know if it is alright to normalize my RNA seq data before mapping the reads to the genome? I ask this because I have the raw file, however the mapping was performed by a bioinformatician who did not provide us the mapped reads file but just the output from after running all the pipelines.
Also, can I run a differential gene expression analysis on the raw reads - even before mapping? Would these results be accepted as valid fold changes if performed on the original files?
Thank you
I was wanting to know if it is alright to normalize my RNA seq data before mapping the reads to the genome? I ask this because I have the raw file, however the mapping was performed by a bioinformatician who did not provide us the mapped reads file but just the output from after running all the pipelines.
Also, can I run a differential gene expression analysis on the raw reads - even before mapping? Would these results be accepted as valid fold changes if performed on the original files?
Thank you
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