Hello to everybody.
I am new in this forum so I hope to use it properly.
I am dealing with a whole exome sequencing and I am pretty new in the field of bioinformatic, so I am facing lots of problems.
I started the analysis with the bwa alignment tools and I obtained my SAM and BAM files.
After that I try to run samtools mpileup (after having sorted and filtered my BAM files from not aligned and paired reads) but the software stalls after a while.
Is there a way to check if my SAM or BAM files are ok?
Or do you have any other suggestion of pipelines more suitable for whole exome sequencing?
Thank you all.
I am new in this forum so I hope to use it properly.
I am dealing with a whole exome sequencing and I am pretty new in the field of bioinformatic, so I am facing lots of problems.
I started the analysis with the bwa alignment tools and I obtained my SAM and BAM files.
After that I try to run samtools mpileup (after having sorted and filtered my BAM files from not aligned and paired reads) but the software stalls after a while.
Is there a way to check if my SAM or BAM files are ok?
Or do you have any other suggestion of pipelines more suitable for whole exome sequencing?
Thank you all.
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