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  • BWA --> SAM: sequence shown of strand where it maps shown

    Hi,

    I mapped some Illumina 75x2 reads using bwa (Version: 0.5.8). When I used samtools pileup I noticed that I have loads os variant calls everywhere. Upon further investigation I noticed that the nt-sequence in the sam file is presented on the strans where the read maps and not on the forward strand of the reference (as it should be?).

    I have not had this problem with pileups using any other aligner. Is this common behavior for bwa? If so, does anyone know what can be done?
    Am I missing something here?

    Thanks
    Last edited by Calle; 10-13-2010, 02:48 PM.

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