What happens try to amp/lin/block/hyb paired end libraries on a single read flowcell?

Dr. Hybe · 02-04-2011, 10:07 AM

Are the adapters the same or will this make a difference. Thanks

hoisinjl · 02-04-2011, 10:19 AM

Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.

gro-seeker · 02-08-2011, 07:23 PM

Quote:

Originally Posted by hoisinjl

Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.

What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?

It looks to me like the adapter sequences are different, and that it wouldn't work the other way around.

Any thoughts?

kmcarr · 02-09-2011, 04:46 AM

Quote:

Originally Posted by gro-seeker

What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?

No, libraries prepared with single read kits are not compatible with paired end cluster generation kits and flowcells.

gro-seeker · 02-09-2011, 09:47 AM

All right, thanks so much! We'll have to go with single reads then.

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02-04-2011, 10:07 AM	#1
Dr. Hybe Member Location: Cambridge Join Date: Jun 2009 Posts: 14	What happens try to amp/lin/block/hyb paired end libraries on a single read flowcell? Are the adapters the same or will this make a difference. Thanks

02-04-2011, 10:19 AM	#2
hoisinjl Member Location: St. Louis, MO Join Date: Jun 2009 Posts: 23	Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.

02-09-2011, 09:47 AM	#5
gro-seeker Junior Member Location: Oakland Join Date: Dec 2010 Posts: 2	All right, thanks so much! We'll have to go with single reads then.