Hi there and thanks in advance for your input!
We extracted total RNA from (plant) leaves, using the quiagen RNeasy kit. As expected, we isolated a lot of plastid rRNA that also shows up in the electropherograms from agilent's bioanalyzer out.pdf. IMHO the traces look fine and do not show signs of degradation, but why do we get such low RINs? Could the plastid rRNAs be the reason for that?
We plan to RNAseq those samples (we aim for mRNA) and I was wondering if the high amount of rRNA will interfere with the polyA library prep?
We extracted total RNA from (plant) leaves, using the quiagen RNeasy kit. As expected, we isolated a lot of plastid rRNA that also shows up in the electropherograms from agilent's bioanalyzer out.pdf. IMHO the traces look fine and do not show signs of degradation, but why do we get such low RINs? Could the plastid rRNAs be the reason for that?
We plan to RNAseq those samples (we aim for mRNA) and I was wondering if the high amount of rRNA will interfere with the polyA library prep?
Comment