|
|
|
|
|||||||
Similar Threads
|
||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| RNA-Seq: De novo transcriptome assembly of RNA-Seq reads with different strategies. | Newsbot! | Literature Watch | 0 | 01-10-2012 05:00 AM |
| PubMed: Monoclonal antibodies isolated without screening by analyzing the variable-ge | Newsbot! | Literature Watch | 0 | 12-24-2010 12:04 PM |
| RNA-Seq: From RNA-seq reads to differential expression results. | Newsbot! | Literature Watch | 0 | 12-24-2010 03:13 AM |
| RNA-Seq: MapSplice: Accurate mapping of RNA-seq reads for splice junction discovery. | Newsbot! | Literature Watch | 2 | 10-14-2010 09:35 AM |
| Redundant reads are removed from ChIP-seq, what about RNA-seq? | asiangg | Bioinformatics | 11 | 08-26-2010 12:10 PM |
 |
|
|
Thread Tools |
10-11-2010, 04:37 PM
|
#1 |
|
Member
Location: CA Join Date: Nov 2008
Posts: 34
|
should RNA-seq use monoclonal reads
hi, guys, I was wondering what's your take on this. Tophat will accept reads that are mapped up to 20 locations and take a fraction for each location. Well, this could be helpful to identify homologous genes, but also create a lots artifact. Do you think we should stay with reads that mapped only once in the genome?
|
|
|
|
10-12-2010, 01:17 AM
|
#2 |
|
Senior Member
Location: Stockholm, Sweden Join Date: Feb 2008
Posts: 319
|
That's not "monoclonal" reads, but rather "multi-mapping" reads.
Personally I think it is too restrictive to require unique mappings in RNA-seq. It's a matter of taste as well as organism + application. |
|
|
|
|
| Thread Tools | |
|
|
