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Two recent articles suggest that CRISPR/Cas9 can cause mutations in the p53 pathway, irrespective of the target sequence of the guide RNA. The data are extremely interesting since they raise the issue of unpredictable off-target effects that are not directly introduced by the CRISPR/Cas9 complex but rather selected during the genome manipulation process. A thorough analysis of the genomic integrity of cells genetically manipulated with CRISPR is, therefore, the key to successful use of the technology, as described in https://perkinelmer-appliedgenomics.com/research-review-crispr-cas9/ .
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM