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  • ABRF Study to compare accuracy and reproducibility of DNA/RNA quantification methods

    This year, the DNA Sequencing Research Group (DSRG) of the ABRF is conducting a multisite study to better understand what methods labs are using to quantify nucleic acids and to discover best practices. Quantifying RNA and DNA samples is one of the most basic methods used in all sequencing and microarray labs. However, beyond the manufacturers’ specifications, there are few standards to tell us how our instruments are performing relative to their optimum behavior, or to each other. We are hoping to learn how prevalent different technologies are, how much their performance varies from location to location, and to learn how well each instrument preforms. As a first step, we have posted an informational survey at https://www.surveymonkey.com/s/YZTDJY5. If you would be interested in participating in the study, we invite you to fill out the survey or to send me a PM.

    The Association of Biomolecular Resource Facilities (ABRF) is dedicated to advancing core and research biotechnology laboratories through research, communication, and education. ABRF members include over 650 scientists representing 360 institutions world wide, including those in industry, government, academic and research institutions. The ABRF is not affiliated with any commercial supplier of instruments, services or supplies.

    Web survey powered by SurveyMonkey.com. Create your own online survey now with SurveyMonkey's expert certified FREE templates.


    Thank you!
    -Stuart Levine & and the rest of the ABRF DSRG

Latest Articles

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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