Dear all,
I measured DNA concentration with two way, QuBit and Epoch.
For NimbleGen exome capture in Illumina sequencing, DNA is needed 30ng/ul.
But DNA concentration measured by Epoch (900ng/ul) was way higher than DNA measured by Qubit(700 ng/ul). I used DNA BR kit for QuBit.
Is Qubit more reliable? I assumed that epoch cannot detect low concentration well.
I measured DNA concentration with two way, QuBit and Epoch.
For NimbleGen exome capture in Illumina sequencing, DNA is needed 30ng/ul.
But DNA concentration measured by Epoch (900ng/ul) was way higher than DNA measured by Qubit(700 ng/ul). I used DNA BR kit for QuBit.
Is Qubit more reliable? I assumed that epoch cannot detect low concentration well.
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