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Old 03-27-2018, 07:32 AM   #1
apeters6
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Location: Boston

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Question Streptavidin Bead Quantitation

Hi Everyone!

I am trying to evaluate how well our new robot handles bead clean-ups with streptavidin beads. I would like to objectively quantitate bead carry over (after removal of supernatant) rather than just visually assessing. Any ideas?

Thanks!
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Old 03-27-2018, 01:36 PM   #2
nucacidhunter
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Counting with a hemocytometer.
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Old 04-01-2018, 07:12 PM   #3
austinso
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more context is needed, but you could:

1. use A600 as a measure of particulate carryover within the supernatant
2. pre-bind beads with biotin-fluorescein, then use biotin to displace to get carryover in terms of biotin-binding equivalents using a standard curve (fluorescence reader required)
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automation, beads, pipetmax, purification, streptavidin

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