I am planning to prepare some RNA-Seq libraries using RNA derived from whole blood (Bos taurus) extracted using the Tempus system from Applied Biosystems. I am concerned that the libraries will contain a large number of transcripts derived from globin mRNA. I have two questions
1 Does anyone have an estimate of the percentage of transcripts that will align to globin?
2 Does anyone have any opinions/advice on the best way to remove the globins either using a globin specific kit or a DNS method.
All advice welcomed.
1 Does anyone have an estimate of the percentage of transcripts that will align to globin?
2 Does anyone have any opinions/advice on the best way to remove the globins either using a globin specific kit or a DNS method.
All advice welcomed.
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