Hi all,
Is there a way to set up NCBI online BLAST to look for sequences that are more distantly related to a subject sequence and have them show up within the top 100? I tried tweaking some settings I saw but seemed to only get very close matches. I am designing a PCR to amplify a family of proteins that is pretty well conserved, but would like to make sure it doesn't amplify something that is outside of acceptable homology.
Thank you!
Is there a way to set up NCBI online BLAST to look for sequences that are more distantly related to a subject sequence and have them show up within the top 100? I tried tweaking some settings I saw but seemed to only get very close matches. I am designing a PCR to amplify a family of proteins that is pretty well conserved, but would like to make sure it doesn't amplify something that is outside of acceptable homology.
Thank you!
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