Hi everybody,
I try to amplify two long-range product about 11000bp each (one amplify exon 1-7 and one amplify exon 19-27), %GC about 62. All criteria for primer design is suitable except primer F for product one located in an region of Loc105371566 gene and primer R for product two is near DNA/TcMar-Tiger. I don't know whether these sequence affect my primer activity. Do anyone have experience about amplify product like this?
I try to amplify two long-range product about 11000bp each (one amplify exon 1-7 and one amplify exon 19-27), %GC about 62. All criteria for primer design is suitable except primer F for product one located in an region of Loc105371566 gene and primer R for product two is near DNA/TcMar-Tiger. I don't know whether these sequence affect my primer activity. Do anyone have experience about amplify product like this?
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