Hello,
We have made TruSeq RNA and DNA libraries for projects sequencing different species and after the bioinformatics QC, the analysis is showing up sequence from species that really shouldn't be there like zebra fish, salmon, and human.
As this has happened over different projects over the past eight months, we don't understand why these same specific species have been showing up across libraries.
Is there a different type of analysis that can be done as part of the QC pipeline to avoid well-sequenced reference genomes like that of the zebra fish? Is there something in these specific genomes that is similar and will interfere with preps/analyses/etc? All of the projects this has occurred in were de novo. Would this affect non-de novo projects in a similar way? Does anyone have any experience of this?
Thanks,
Anna.
We have made TruSeq RNA and DNA libraries for projects sequencing different species and after the bioinformatics QC, the analysis is showing up sequence from species that really shouldn't be there like zebra fish, salmon, and human.
As this has happened over different projects over the past eight months, we don't understand why these same specific species have been showing up across libraries.
Is there a different type of analysis that can be done as part of the QC pipeline to avoid well-sequenced reference genomes like that of the zebra fish? Is there something in these specific genomes that is similar and will interfere with preps/analyses/etc? All of the projects this has occurred in were de novo. Would this affect non-de novo projects in a similar way? Does anyone have any experience of this?
Thanks,
Anna.
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