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Old 01-17-2011, 06:46 AM   #1
Inti
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Location: Mexico city

Join Date: Jul 2008
Posts: 12
Default RNA-seq for differential expression

Hello!
We would like to start some RNA-seq experiments for quantitation and differential expression but I am not sure how deep should we sequence a sample to have enough coverage and have a good estimation of the transcripts
meaning: Should we sequence 1 or 2 lanes per sample or can we multiplex several samples in one lane?
This point is crucial since we have several samples and cost is very important!
The organism is Mouse and we have a GA IIx.
Has anybody done this kind of experiments that can share some advice?
Thanks!
Inti
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