Hi all,
this is my first post here
And as slowly entering in deep-seq world I was wandering how to use a data in GEO datasets NCBI, for Example that one from Illumina:
Is downloading "full table" will dive me an untrimmed from adaptors pure reads ready for mapping or?
At the bottom they provide fastaq format but I think is untrimmed? and how to trimm adaptors if I do not know the adaptors seq?
Can I just "Download full table..." and map the seqs to reference genome?
As a finale goal I want/need to generate a GFF file with locations of these small RNAs
sorry for my newbies questions but I feel like a pin in the ocean of info
Thanks!
this is my first post here
And as slowly entering in deep-seq world I was wandering how to use a data in GEO datasets NCBI, for Example that one from Illumina:
Is downloading "full table" will dive me an untrimmed from adaptors pure reads ready for mapping or?
At the bottom they provide fastaq format but I think is untrimmed? and how to trimm adaptors if I do not know the adaptors seq?
Can I just "Download full table..." and map the seqs to reference genome?
As a finale goal I want/need to generate a GFF file with locations of these small RNAs
sorry for my newbies questions but I feel like a pin in the ocean of info
Thanks!