Hey everyone,
I hope someone can help . I'm trying to preform amplicon sequencing on the Miseq. I'm using a custom target specific primer with the linker adapter to then follow the Illumina 16S library prepartion protocol. I'm struggling with the sensitivity of the inital PCR which isn't comparable with the level I managed to achieve with the target specific primer without the linker . I need to improve the sensitivity as the samples I'm using have very low levels of DNA. Any suggestions on how to improve would be great.
I hope someone can help . I'm trying to preform amplicon sequencing on the Miseq. I'm using a custom target specific primer with the linker adapter to then follow the Illumina 16S library prepartion protocol. I'm struggling with the sensitivity of the inital PCR which isn't comparable with the level I managed to achieve with the target specific primer without the linker . I need to improve the sensitivity as the samples I'm using have very low levels of DNA. Any suggestions on how to improve would be great.
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