Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Viral genome sequencing

    I want to perform whole viral genome sequencing on ION PGM and I donot have specific primers for it.
    Are this kit ( Ion Total RNA-Seq Kit for AB Library Builder™ System cat no.4482416) compatible for my application ?

  • #2
    It is marketed as the automated version of the Ion Total RNA-Seq Kit v2, which is compatible with viral transcriptomes.
    But you're going to make Thermo a call anyways, to correctly gauge the quantity of other reagents... so you might as well ask them directly, and be sure.

    Comment


    • #3
      Originally posted by r.rosati View Post
      It is marketed as the automated version of the Ion Total RNA-Seq Kit v2, which is compatible with viral transcriptomes.
      But you're going to make Thermo a call anyways, to correctly gauge the quantity of other reagents... so you might as well ask them directly, and be sure.
      Dear/rosati
      Thanks for your care and reply
      I know this kit (Ion Total RNA-Seq Kit v2), but this specific for manual library and I have automated library builder that require ( Ion Total RNA-Seq Kit for AB Library Builder™ System cat no.4482416)
      But in case of these two kit , a big problem will face me that the host RNA will interfer with the viral RNA and this will make big noisy in my data in analysis stage.
      if you know solution for my problem ,Please contact on my email: [email protected]
      Thanks in advance
      Best Regards
      Hnay

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      7 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      7 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      49 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      66 views
      0 likes
      Last Post seqadmin  
      Working...
      X