Hello SEQanswers,
I'm considering a MiSeq for verification of newly assembled plasmids (<10kb per plasmid). Obviously, I'd like to be able to multiplex many plasmids per run.
I know they sell a Nextera kit with 12 barcodes. Has anyone multiplexed ~96 samples together during Nextera sample prep? If so, how much of a pain was it?
Thanks!
Barry
I'm considering a MiSeq for verification of newly assembled plasmids (<10kb per plasmid). Obviously, I'd like to be able to multiplex many plasmids per run.
I know they sell a Nextera kit with 12 barcodes. Has anyone multiplexed ~96 samples together during Nextera sample prep? If so, how much of a pain was it?
Thanks!
Barry
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