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Old 12-18-2014, 06:48 AM   #1
Location: /home/bob

Join Date: Jun 2012
Posts: 59
Default DESeq2 ntop set to 500?

Hi all,

I was just making some PCAs and I noticed that the plotPCA function within DESeq2 only takes the 500 genes with the highest variance. I was wondering if anyone knows what is the justification for this? vs taking the whole dataset? Is it just an attempt to filter out noise?

I'm working on a mouse RNA-Seq dataset at the moment and the plots look quite different if I take 500 genes vs taking 1000 genes
bob-loblaw is offline   Reply With Quote

deseq2, pca plot

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