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Old 04-30-2012, 04:54 AM   #1
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Location: Purdue University, West Lafayette, Indiana

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Default Worst MiSeq Run ever?

In a big hurry to get our second (non-validation) run on our MiSeq. Got the wrong dilution of the library and loaded it at 250 pM (instead of 10 pM or so.)

I am sure many have had results just as poor. This is mainly to temper any charges of "hubris" with the HiSeq lane post.

Also, note the reported raw cluster density in the table. Just as a matter of error propagation, one would have preferred "zero" or "20000" Kcluster/mm^2 for the raw cluster number. Although the latter presumes a flow cell could actually support such a cluster density and, even less likely, that software would be able to find all these tiny clusters.

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