SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Stopped MiSeq run memento Illumina/Solexa 24 10-28-2015 07:17 PM
MiSeq run failure - inhibition? firefly2280 Illumina/Solexa 24 11-14-2014 07:13 PM
MiSeq stopped run yaximik Illumina/Solexa 20 03-18-2013 07:10 AM
How much PhiX in MiSeq run? thdybwf Illumina/Solexa 1 11-26-2012 03:01 AM
Worst MiSeq Run ever? pmiguel Illumina/Solexa 0 04-30-2012 04:54 AM

Reply
 
Thread Tools
Old 07-01-2015, 11:13 AM   #1
microgirl123
Senior Member
 
Location: New England

Join Date: Jun 2012
Posts: 196
Default Less than 2 nM for a MiSeq run?

I'm sure this has been posted here before, but I can't find it!

I've got some gDNA libraries that are coming in as less than 2 nM with qPCR quantification after PCR enrichment. I know there's a way to adjust the MiSeq denaturation/dilution/loading protocol to allow for a lower starting concentration. Does anyone do this or remember the thread this info was posted in?
microgirl123 is offline   Reply With Quote
Old 07-01-2015, 02:38 PM   #2
HESmith
Senior Member
 
Location: Bethesda MD

Join Date: Oct 2009
Posts: 503
Default

http://seqanswers.com/forums/showthr...dilute+samples
HESmith is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:33 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO