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Old 07-01-2015, 11:13 AM   #1
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Default Less than 2 nM for a MiSeq run?

I'm sure this has been posted here before, but I can't find it!

I've got some gDNA libraries that are coming in as less than 2 nM with qPCR quantification after PCR enrichment. I know there's a way to adjust the MiSeq denaturation/dilution/loading protocol to allow for a lower starting concentration. Does anyone do this or remember the thread this info was posted in?
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Old 07-01-2015, 02:38 PM   #2
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