hi,
I am doing de novo assembly of 454 reads, i have got the contigs using mira assembler, now i want to do the scaffolding of those contigs, the scaffolding tool that comes with mira is bambus but it uses reference genome for scaffolding, some one please suggest me how to perform the scaffolding of the contigs without having reference sequence also my data is not paired end.
I am doing de novo assembly of 454 reads, i have got the contigs using mira assembler, now i want to do the scaffolding of those contigs, the scaffolding tool that comes with mira is bambus but it uses reference genome for scaffolding, some one please suggest me how to perform the scaffolding of the contigs without having reference sequence also my data is not paired end.
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