Is there any drawbacks of using Illumina or Solid to sequence BAC clones?
A company quoted about 10K to sequence one BAC clone (about 140Kb) using the conventional way (shotgun-sanger-assembly), but I thought that I could multiplex 12 different clones in a single Illumina lane and get them all done by half of that money. Am I missing something?
Cheers
A company quoted about 10K to sequence one BAC clone (about 140Kb) using the conventional way (shotgun-sanger-assembly), but I thought that I could multiplex 12 different clones in a single Illumina lane and get them all done by half of that money. Am I missing something?
Cheers
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