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  • bfast postprocess error

    Dear users,
    I'm running a file fastq of microRNA reads with bfast, using as reference the microRNA precursor sequence.

    The step "localalign" runs only if I set the parameter -u (Do ungapped local alignment).

    I run the bfast postprocess step as:
    >bfast postprocess -f hsa_human_miRNAprec_miRBaseV13.fasta -i sample_622T_9.baf -a 3 -O 3 -o sample

    but it returns me an error and it crashes:

    In function "GetReverseComplimentAnyCaseBase": Fatal Error[OutOfRange]. Message: Could not understand sequence base.

    Has someone had the same problems?
    Thanks very much!
    Bye bye,
    Maria Elena

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