Given the cutting frequency of NlaIII and DpnII, why aren't they used for generating libraries from genomic DNA? I realize that they were originally used to obtain sequence tags for expression, but haven't seen them used for preparing genomic libraries. why not?
in general, why would you not use restriction enzymes for preparing genomic libraries? Has anyone looked at this systematically recently?
in general, why would you not use restriction enzymes for preparing genomic libraries? Has anyone looked at this systematically recently?
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