Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Small RNA Library Sequencing on NextSeq jteeee2 RNA Sequencing 4 06-23-2016 10:58 PM
NextSeq Data Brian Bushnell Illumina/Solexa 7 07-22-2015 02:48 PM
Pippin -- forgot to cover elution wells! maierpa Sample Prep / Library Generation 0 04-07-2014 09:38 PM
heat denature or not? ilma 454 Pyrosequencing 4 03-28-2012 07:48 AM
10ul of 700pM library. How to Denature and cluster? yoyoming1001 Illumina/Solexa 2 11-02-2010 06:05 AM

Thread Tools
Old 07-31-2018, 03:08 PM   #1
Junior Member
Location: san diego

Join Date: Jul 2018
Posts: 2
Default Forgot to denature NextSeq library

I forgot to heat denature my NextSeq500 library. Will it still cluster and give enough data or should I cancel the run?
kvanderlinden is offline   Reply With Quote
Old 07-31-2018, 04:31 PM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 354

It should not cluster at all. Please let us know in case you witness a miracle.
luc is offline   Reply With Quote

denature, nextseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:13 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO