Hi all
I'm running a microbiome analysis of fish gut microdlora using Illumina MiSeq 16s pipeline. I'd like to get your thoughts on my indexing PCR gel - some of the bands seem a little faint. Can band strength be an indicator of performance of the samples once pooled and sequenced?
Gel pic attached.
Would really appreciate any insights.
Thank you
Egansbay
I'm running a microbiome analysis of fish gut microdlora using Illumina MiSeq 16s pipeline. I'd like to get your thoughts on my indexing PCR gel - some of the bands seem a little faint. Can band strength be an indicator of performance of the samples once pooled and sequenced?
Gel pic attached.
Would really appreciate any insights.
Thank you
Egansbay
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