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  • Looking for assembler to align very short reads

    Hi all,

    Now I have a question, when I tried to align several very short length of reads, I used cap3, it cannot get the consensus sequence. The input file list below:
    >S3O
    CCGAGGCACACACAGCGCGAGTGGGCAAGGCGTGGCTGGGAACGTAGGGGTCTCAG
    >6EC
    CATGTTCTTTGAAGGTACTTCCCAAGAAAGTTACATTTAGCACGTAAACAAAACAAGCACCTCCAATATTC
    >86V
    ACTTGGATGTCGACCGAAGCTGGGAAACCATCCTATACGAAAAATGACTCGCCGTCCATCTCCGAGGCACAC
    >X0L
    ACTTGGATGTCGACCGAAGCTGGGAAACCATCCTATCGAAAAATGACTCGCTGTCCATCTCCAAGGCACAC
    >67T
    CTTGGATGTCGACTGAAGCTGGGAAACCATCCTATCACAACATGACGTGCCGGCCATCTCCAAGGCACA

    Obviously, there is one part sequence 'GGGAAACCATCCTATACGAAAAA' can be align from above sever reads. However, when I run it on cap3, it get nothing. Any one can recommend a better assembler? I expect to get the .ace file as out.
    Last edited by andylai; 06-02-2011, 09:04 PM.

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