Hello forumers,
I am currently trying to find out if it's possible to run a bioanalyzer (Agilent 2100) on samples containing DNA/RNA duplexes.
So I have performed a cDNA synthesis on some rRNA and now I want to measure the size of the cDNA/rRNA hybrid fragments. I guess if I would use the RNA kit it would be impossible to distinguish free rRNA in my sample from the DNA/RNA fragments, but what if I use a DNA kit? I shouldn't have any DNA before the cDNA synthesis so in theory that should give me some information about the DNA/RNA hybrids right?
Or do you think I should do a second strand synthesis of my DNA/RNA (get rid of the RNA and replace with DNA) in order to get dsDNA and then use a DNA kit for analyzing?
I'm not very familiar with the exact chemistry behind the dyes of the different bioanalyzer kits and I had a hard time to find this information on the web.
Thank you in advance
/Koncarius
I am currently trying to find out if it's possible to run a bioanalyzer (Agilent 2100) on samples containing DNA/RNA duplexes.
So I have performed a cDNA synthesis on some rRNA and now I want to measure the size of the cDNA/rRNA hybrid fragments. I guess if I would use the RNA kit it would be impossible to distinguish free rRNA in my sample from the DNA/RNA fragments, but what if I use a DNA kit? I shouldn't have any DNA before the cDNA synthesis so in theory that should give me some information about the DNA/RNA hybrids right?
Or do you think I should do a second strand synthesis of my DNA/RNA (get rid of the RNA and replace with DNA) in order to get dsDNA and then use a DNA kit for analyzing?
I'm not very familiar with the exact chemistry behind the dyes of the different bioanalyzer kits and I had a hard time to find this information on the web.
Thank you in advance
/Koncarius
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