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  • Poly A cDNA- 454 Titanium

    Hi
    My name is Amir. I just finished my first run on the Titanium 454 using polyA cDNA as atemplate. The output was quite low around 100Mb and contain large stretches of Ts.
    I will be happy tp get responsese abput the possibility to run PolyA cDNA on the Titanium. The facilty I used claim that there is no problem running polyA cDNA on the Titanium but it seems that most places do not do it.
    Thanks
    Amir

  • #2
    Stretches of T in cDNA reads cause problems during sequencing, as they result in a large flash of light. Neighboring wells are influenced by this and might fail, causing a reduced run output.

    Best to avoid the polyA tail next time...
    And, perhaps switch sequencing provider.

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