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We have recently been experiencing problems with the amplifcation manifolds from Illumina in their ability to form a tight seal on the cluster station. The result has been decreased reagent flow and very low cluster density when sequencing. Has anyone else been experiencing this issue and has anyone found ways to fix the problem other than contacting Illumina directly? Thanks.
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We experienced the similar problem before.
Try to tighten fluid tubes against the rubber adaptors before using the manifold, it will help. We observed those connection were sometimes not tight enough and the cluster station will pump air occasionally. By tighten the connection manually, we solved the problem.
We have a cBot now, haven't experienced the same problem since then.
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