Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • DEXSeq splicing plot from CuffDiff output

    I am new to RNASeq and just followed Nature protocol paper for Tophat-Cufflink for analysis (http://www.nature.com/nprot/journal/....2012.016.html).
    Now I want to get alternative splicing visualization that is not described in that paper. I don't know how to do that or better tool for that. I came across DEXSeq and manual shows similar plots but have no Idea how to do so from my analysis output or data.
    Please help me to get those type of graphs as shown on Page 10 on DEXSeq manual at http://watson.nci.nih.gov/bioc_mirro...doc/DEXSeq.pdf

    Highly appreciate your help.

  • #2
    If you want to use the plotting function of DEXSeq, the vignette that you posted has the instructions to make them. You have to make an ExonCountSet object with your data, you can find an example of how to do this in the pasilla data package vignette:

    The Bioconductor project aims to develop and share open source software for precise and repeatable analysis of biological data. We foster an inclusive and collaborative community of developers and data scientists.


    Your idea, I guess, would be to use plotDEXSeq in the cuffdiff hits.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 08:47 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    59 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    54 views
    0 likes
    Last Post seqadmin  
    Working...
    X