Hi,
has anyone used Tophat to look at long non coding RNAs or mRNA like ncRNAs using NGS?
I am assuming of course that things would be easier if I had 454 or even Sanger reads. But Solexa and SOLiD would be more bang for the buck.
aside from de novo assembly of the transcriptome. I am wondering if it is possible to use Tophat / Cufflinks to generate the transcripts
has anyone used Tophat to look at long non coding RNAs or mRNA like ncRNAs using NGS?
I am assuming of course that things would be easier if I had 454 or even Sanger reads. But Solexa and SOLiD would be more bang for the buck.
aside from de novo assembly of the transcriptome. I am wondering if it is possible to use Tophat / Cufflinks to generate the transcripts
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