Hi everyone,
I downloaded Illumina data from NCBI short read sequencing archive. I used script located http://maq.sourceforge.net/fq_all2std.pl to conver the data to fasta format. The converted fasta data is having N character including regular DNA characters like A,C,G and T.
My assembler runs only with A,C,G and T. Is the conversion correct? Is there any additional script to be run to get rid of N character.
Thanks
Bala
I downloaded Illumina data from NCBI short read sequencing archive. I used script located http://maq.sourceforge.net/fq_all2std.pl to conver the data to fasta format. The converted fasta data is having N character including regular DNA characters like A,C,G and T.
My assembler runs only with A,C,G and T. Is the conversion correct? Is there any additional script to be run to get rid of N character.
Thanks
Bala
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