Homemade illumina oligos and library prep kit

elly · 12-01-2008, 07:20 PM

Hi,

I am wondering if we can use the PCR amplification primers which were made at our side.
In theory, this can work properly.

But I am worrying a little bit if there can be some problem with sequencing primer binding and PCR amplification step.

Is there anyone who attempt this?

Thank you in advance.

graveley · 01-24-2009, 10:39 AM

Yes, as long as the sequences you use are the same as the Illumina sequences, primers you synthesize yourself work fine!

andibody · 02-02-2009, 08:04 AM

Is this also true for adaptors and sequencing primers?

Thanks
Andi

kkannan · 03-24-2009, 08:05 AM

For the PE PCR primers, is it necessary to PAGE purify them?

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12-01-2008, 07:20 PM	#1
elly Junior Member Location: K Join Date: Apr 2008 Posts: 5	Homemade illumina oligos and library prep kit Hi, I am wondering if we can use the PCR amplification primers which were made at our side. In theory, this can work properly. But I am worrying a little bit if there can be some problem with sequencing primer binding and PCR amplification step. Is there anyone who attempt this? Thank you in advance.

01-24-2009, 10:39 AM	#2
graveley Member Location: Hartford, CT Join Date: Jan 2009 Posts: 11	Yes, as long as the sequences you use are the same as the Illumina sequences, primers you synthesize yourself work fine!

02-02-2009, 08:04 AM	#3
andibody Member Location: Vienna Join Date: Mar 2008 Posts: 45	Is this also true for adaptors and sequencing primers? Thanks Andi

03-24-2009, 08:05 AM	#4
kkannan Junior Member Location: Houston Join Date: Jan 2009 Posts: 1	For the PE PCR primers, is it necessary to PAGE purify them?